Article summary
Genome-wide, targeted loss-of-function pooled
screens using the clustered, regularly interspaced, short palindromic
repeats (CRISPR)-associated nuclease Cas9 in human and mouse
cells provide an alternative screening system to RNA interference
(RNAi). Previously, we used a genome-scale CRISPR knockout
(GeCKO) library to identify loss-of-function mutations conferring
vemurafenib resistance in a melanoma model1. However, initial lentiviral
delivery systems for CRISPR screening had low viral titer or
required a cell line already expressing Cas9, thereby limiting the
range of biological systems amenable to screening.
Reference: Sanjana, Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors and genome-wide libraries for CRISPR screening." Nature methods 11.8 (2014): 783-784.
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