The Cas9 endonuclease is the central component of the Type II CRISPR/Cas system, a prokaryotic adaptive restriction system against invading nucleic acids, such as those originating from bacteriophages and plasmids. Recently, this RNA-directed DNA endonuclease has been harnessed to target DNA sequences of interest. Here, Drs Sampson, Timothy R.and David S. Weiss reviewed the development of Cas9 as an important tool to not only edit the genomes of a number of different prokaryotic and eukaryotic species, but also as an efficient system for site-specific transcriptional repression or activation. Additionally, a specific Cas9 protein has been observed to target an RNA substrate, suggesting that Cas9 may have the ability to be programmed to target RNA as well. Cas proteins from other CRISPR/Cas subtypes may also be exploited in this regard. Thus, CRISPR/Cas systems represent an effective and versatile biotechnological tool, which will have significant impact on future advancements in genome engineering.
Reference: Sampson, Timothy R., and David S. Weiss. "Exploiting CRISPR/Cas systems for biotechnology." Bioessays 36.1 (2014): 34-38.
Information is nice and good but it seems to be something is missing.all the categories got the same specifications and it still requires some more new things to be shown to the world.
ReplyDeletegRNA synthesis