Protocol : Improved vectors and genome-wide libraries for CRISPR screening

Article summary Genome-wide, targeted loss-of-function pooled screens using the clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated nuclease Cas9 in human and mouse cells...
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Genome-engineering with CRISPR-Cas9 in the mosquito Aedes aegypti

Article summary The mosquito Aedes aegypti is a potent vector of the Chikungunya, yellow fever, and Dengue viruses, which result in hundreds of millions of infections and over 50,000 human deaths...
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Protocol : A CRISPR/Cas9 toolkit for multiplex genome editing in plants

Article summary Background To accelerate the application of the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/ CRISPR-associated protein 9) system to a variety of plant...
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COSMID: A Web-based Tool for Identifying and Validating CRISPR/Cas Off-target Sites

Article summary Precise genome editing using engineered nucleases can significantly facilitate biological studies and disease treatment. In particular, clustered regularly interspaced short palindromic...
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An Active Immune Defence with a Minimal CRISPR (clustered regularly interspaced short palindromic repeats) RNA and Without the Cas6 Protein

Article summary The prokaryotic immune system CRISPR-Cas1 is a defence system that protects prokaryotes against foreign DNA. The short CRISPR RNAs (crRNAs) are central components of this immune...
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Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery

Article summary The CRISPR/Cas9 system is a robust genome editing technology that works in human cells, animals and plants based on the RNA-programmed DNA cleaving activity of the Cas9 enzyme....
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